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SPECIES: mouse, BALB/c
ECACC catalog code: 90031902
TRANSFORMED BY: Moloney-MSV
Description: Mouse BALB/c embryonic fibroblast, M-MSV transformed
Viruses: See Properties
Depositor: Dr C Chany, INSERM, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Interferon, transformation and virus studies: M-MSV
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM(EBSS) + 1% Non Essential Amino Acids (NEAA) or RPMI 1640 + 2mM Glutamine + 50-100 IU/ml murine IFN + 5-10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from the MSV.B line (ECACC No. 90031901) which has been exposed to 50 IU/ml Interferon for over 200 passages. Unlike the parent line, the cells are Non-malignant and do Not form tumours in nude mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cancer Res 1989;49:1241 - PMID: 2521813
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293