MOLT-3

Version 4.0.200811

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MOLT-3 (human, peripheral blood, leukemia, T cell)

SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, T cell

DSMZ catalog code: ACC 84
Morphology: round, single cells in suspension
Description: human T cell leukemia established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1971; sister cell line of MOLT-4 (DSM ACC 362) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every second day; seed out at ca. 0.5-0.7 x 106 cells/ml at 37C with 5% CO2 maximal density at about 0.7-1.0 x 106 cells/ml; doubling time of ca. 40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypertetraploid karyotype; 98(94-101)<4n>XXYY, +6, +7, +8, +8, +17, +20, del(6)(q16)x2, der(7)t(7;7)(p15;q11)x2) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD2+ CD3- CD4- CD5+ CD6+ CD7+ CD8+ CD13- TCR_/beta-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative for reverse transcriptase, HLTV-I/II, HIV-1)

Availability in cell line catalogues: ATCC: CRL 1552, IZSBS: BS TCL44, ECACC: 90021901

Bibliographic references:

J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231