B3/AN

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

B3/AN (mouse, AKR, thymus, thymoma)

SPECIES: mouse, AKR
TISSUE/ORGAN: thymus
TUMOR: thymoma

ECACC catalog code: 94031009
Morphology: Spherical
Description: Mouse, AKR, thymus thymoma
Viruses: See Properties
Depositor: Dr D Iannelli, University of Naples, School of Agriculture, ITALY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: To study the assembly and transport of Ly-2/3 antigen to the cell surface or to define the regions of Ly-2/3 interacting with the corresponding antibodies and with MHC Class I molecules.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS). Maintain cultures between 3-9x100,000 cells/ml; 5-10% CO2; 37C. Saturation density is reached at 2x1,000,000 cells/ml.
This Ly-2/3 negative cell line was derived from the parent cell line AKRI.G.1 OUAR.1.26
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ICLC: ATL97004

Bibliographic references:

Immunogenetics 1994;40:154 - PMID: 7517914
J Immunol Methods 1992;154:211 - PMID: 1401954