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SPECIES: mouse, AKR
ECACC catalog code: 94031009
Description: Mouse, AKR, thymus thymoma
Viruses: See Properties
Depositor: Dr D Iannelli, University of Naples, School of Agriculture, ITALY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: To study the assembly and transport of Ly-2/3 antigen to the cell surface or to define the regions of Ly-2/3 interacting with the corresponding antibodies and with MHC Class I molecules.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS). Maintain cultures between 3-9x100,000 cells/ml; 5-10% CO2; 37C. Saturation density is reached at 2x1,000,000 cells/ml.
This Ly-2/3 negative cell line was derived from the parent cell line AKRI.G.1 OUAR.1.26
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: ATL97004
- Immunogenetics 1994;40:154 - PMID: 7517914
- J Immunol Methods 1992;154:211 - PMID: 1401954
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293