MDCK

Version 4.0.200811

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MDCK (dog, cocker spaniel, kidney)

IZSBS catalog code: BS CL 64
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: dog, cocker spaniel (female) Karyology: 2n = 78 in 100 cells
TISSUE/ORGAN: kidney
Susceptibility to viruses: adenovirus 4 * adenovirus 5 * canine distemper virus * canine hepatitis * Coxsackie B5 * influenza A * influenza B * reovirus 2 * reovirus 3 * swine fever virus * swine vesicular exanthema virus * vaccinia * vesic. stomatitis (Indiana)
DEPOSITOR: obtained from Flow Laboratories, Irvine (GB)
PROPERTIES: expressing xenobiotic metobolizing enzymes; virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-2 Passage: 53, mycoplasma negative, culture
Validated by: isoenzymes (confirmed as dog with NP, G6D, MD, MPI, AST, PEPB)

Availability in cell line catalogues: ATCC: CCL 34, ECACC: 84121903, ECACC: 85011435

Bibliographic references:

Am J Vet Res 1968;29:1033 - PMID: 4967124
Appl Microbiol 1968;16:588 - PMID: 5647517