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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: AL97001
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: mouse, C3H/An (male, 100 days old)
TISSUE/ORGAN: connective
Derived from: L-M (ATCC CCL1.2)
DEPOSITOR: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: resistant to 5-BUdR;thymidine kinase mutant; biochemical and genetic studies, including somatic cell hybridization and gene transfer
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin /EDTA; seed at 5x103-2x104 cells/cm2; 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with AST, G6PD)
Availability in cell line catalogues: ECACC: 85011432
Bibliographic references:
- Cell 1977;11:223 - PMID: 194704
- Exp Cell Res 1963;31:297-312
- Proc Natl Acad Sci USA 1972;69:510-514 - PMID: 4110482
- Proc Natl Acad Sci USA 1980;77:1583-1587 - PMID: 6929511
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it