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SPECIES: mouse, C3H/An
ECACC catalog code: 85011432
Description: Mouse C34/An connective tissue
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Somatic cell hybridization and gene transfer studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Sub-line of BUdR resistant strain of the L-M mouse line, which in turn is derived from L929. TK- cells cannot grow in HAT medium. Stable TK- mutants are only obtained if the cells are kept for several passages in growth medium containing BUdR.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: AL97001
- Cell 1977;11:223 - PMID: 194704
- Exp Cell Res 1963;31:297-312
- J Natl Cancer Inst 1943;4:165-212
- J Natl Cancer Inst 1961;26:1075
- Proc Natl Acad Sci U S A 1972;69:510-514 - PMID: 4110482
- Proc Natl Acad Sci U S A 1972;77:1583-1587
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293