This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, C3H/An
TISSUE/ORGAN: areolar and adipose
ECACC catalog code: 85103115
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Mouse C3H/An areolar and adipose tissue
Viruses: See Properties
Depositor: Mrs M Freshney, The Beatson Institute, Glasgow, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split semi-confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x100,000 cells /cm2 using 0.25% trypsin or trypsin EDTA; 5% CO2; 37C.
Derived from normal subcutaneous connective tissue of an adult C3H strain mouse. The cells were treated with 20 methylcholanthrene and produced sarcomas when injected into C3H strain mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- J Natl Cancer Inst 1948;9:229
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293