|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, C3H/An
TISSUE/ORGAN: connective, fibroblast
DSMZ catalog code: ACC 2
Morphology: fibroblasts growing as monolayer
Susceptibility to viruses: bluetongue virus * herpes simplex * pseudorabies * vesic. stomatitis (Indiana)
Description: mouse connective tissue fibroblast established from the normal subcutaneous areolar and adipose tissue of a male C3H/An mouse; used as target in TNF detection assays confirmed as mouse with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions
Available in the following LABORATORY:
-
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent cultures 1:5 to 1:10 using trypsin (do not use trypsin/EDTA); after 2-3 days monolayer will be confluent; split 2-3 times a week; seed out initially at about 1.0-2.0 x 106 cells/25 cm 2 at 37C with 5% CO2 ca. 4-8 x 106 cells/25 cm 2;
doubling time of ca. 21-24 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, PCR assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, MDH, PEP B)
Bibliographic references:
- J Clin Microbiol 1988;26:2324 - PMID: 2853175
- J Natl Cancer Inst 1943;4:165-212
- J Natl Cancer Inst 1948;9:229
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it