KHOS/NP

Version 4.0.200811

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KHOS/NP (human, Caucasian, bone, osteosarcoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: bone
TUMOR: osteosarcoma

ECACC catalog code: 84102903
Morphology: Epithelial
TRANSFORMED BY: Kirsten MSV
Description: Human Caucasian osteosarcoma
Viruses: See Properties
Depositor: Mr T Littlewood, Ludwig Institute for Cancer Research, Cambridge, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Assay of type-C leukemia viruses.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 3; Isolated from transformed foci of human osteogenic sarcoma (HOS) cells which were induced by Kirsten murine sarcoma virus. They contain MSV genome but do not produce infectious virus. The transformed cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1544, IZSBS: BS TCL80

Bibliographic references:

Int J Cancer 1975;15:23-29 - PMID: 171229
Int J Cancer 1975;16:840 - PMID: 171229