JTC-27

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

JTC-27 (rat, ascitic fluid, hepatoma)

SPECIES: rat
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma

ECACC catalog code: 94042255
Morphology: Epithelial-like
Description: Rat hepatoma
Viruses: See Properties
Depositor: Dr T Ohno, Riken Gene Bank, Tsukuba, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Drug metabolism assays, hepatocyte studies, toxicological studies.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DM-160 AU + 2mM Glutamine + 2% Fetal Bovine Serum (FBS) (10% for initial cell attachment after thawing). Split confluent cultures 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 13; Karyotype: Modal No52
Derived from the rat ascites hepatoma.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ICLC: ATL98013, DSMZ: ACC 330

Bibliographic references:

Blood 1975;45:321-334 - PMID: 163658
J Exp Med 1972;42:249