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TISSUE/ORGAN: ascitic fluid
ECACC catalog code: 94042255
Description: Rat hepatoma
Viruses: See Properties
Depositor: Dr T Ohno, Riken Gene Bank, Tsukuba, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Drug metabolism assays, hepatocyte studies, toxicological studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DM-160 AU + 2mM Glutamine + 2% Fetal Bovine Serum (FBS) (10% for initial cell attachment after thawing). Split confluent cultures 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No52
Derived from the rat ascites hepatoma.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: ATL98013, DSMZ: ACC 330
- Blood 1975;45:321-334 - PMID: 163658
- J Exp Med 1972;42:249
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293