Amdur II

Version 4.0.200811

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Amdur II (human, Caucasian, skin, fibroblast, methylmalonicacidemia)

SPECIES: human, Caucasian
TISSUE/ORGAN: skin, fibroblast
PATHOLOGY: methylmalonicacidemia*251000 (OMIM record)

ECACC catalog code: 90112605
Morphology: Fibroblast
Susceptibility to viruses: herpes simplex * poliovirus 1 * vaccinia * vesic. stomatitis (Indiana)
Description: Human Caucasian skin methylmalonicacidemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Genetic studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 2% Amino Acids + 2% Vitamins + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 18; Karyotype: 2n = 46
Derived from the skin biopsy of a one year-old Caucasian male with methylmalonicacidemia. The cells have a limited lifespan; up to 40 passages are expected.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 124, IZSBS: BS CL2

Bibliographic references:

Pediatr Res 1969;3:217 - PMID: 5788784