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SPECIES: mouse, AKR
ECACC catalog code: 91061307
Description: Mouse, AKR, embryo
Viruses: See Properties
Depositor: Dr H Moses, Vanderbilt University School of Medicine, Tennessee, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Can spontaneously produce murine leukaemia virus (MLV) in prolonged confluent culture. Applications: Culture of murine leukaemia virus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:16 to 1:24 i.e. seeding at 4-10x1,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Do not allow to reach confluence or grow over 20 passages.
This cell line has the capacity to be activated to produce murine leukaemia virus. Mice of the high leukaemic inbred strain AKR regularly contain high levels of MLV throughout most of their post-natal life. However, studies of the AKR mouse embryo indicated that they contain very little, or no, infectious virus. Cell lines were found to produce virus only after several weeks in culture. Virus-negative cell lines can be induced to initiate synthesis of MLV (Virology 1971). Multiple transforming growth factors (TGFs) have been isolated from the serum-free conditioned media and acid-ethanol cell extracts of non-transformed AKR2B cells.
Research council deposit: No;
Price Code: C;
- Cancer Res 1983;43:1581 - PMID: 6299525
- Virology 1971;46:866 - PMID: 4332980
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293