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ECACC catalog code: 86061902
Description: Mouse lymphoblast
Viruses: See Properties
Depositor: Dr J Garland, Department of Immunology, University of Manchester, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Interleukin 3 (IL-3) Applications: Growth factor studies, IL-2 assays
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO2; 37C. Cells will rapidly die at high densities above. 5x100,000 cells/ml. Culture very sensitive to agitation.
Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Lymphokines 1984;9:153
- Proc Natl Acad Sci U S A 1984;81:1208 - PMID: 6422470
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293