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SPECIES: toad, South African
ECACC catalog code: 89072613
Description: South African clawed toad kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Replication studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
NCTC 109 + 15% Distilled H2O + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 27-28C.
Derived from normal kidneys of an adult male toad (xenopus laevis). A6 cells support the replication of Granoff's frog viruses but not the Lucke inclusion-tumour associated virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 102
- Biology of Amphibian Tumours (M Mizell ed, Springer-Verlag) 1969;52-81
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293