A2058

Version 4.0.200811

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A2058 (human, skin, melanoma, metastatic)

SPECIES: human
TISSUE/ORGAN: skin
TUMOR: melanoma, metastatic

ECACC catalog code: 91100402
Morphology: Epithelial
Description: Human caucasian metastatic melanoma
Viruses: See Properties
Depositor: Dr M Simpkins,Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Nerve growth factor (NGF), Laminin

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.
Research council deposit: Yes; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ICLC: HTL08002

Bibliographic references:

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