|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL96006
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Japanese (male, 66 years old) Karyology: modal chromosome number 37
TISSUE/ORGAN: pancreas
TUMOR: adenocarcinoma
Derived from: ascitic fluid
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA production
Available in the following LABORATORY:
-
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:4-1:10 using trypsin/EDTA(10-15 min); seed at 2-4x104 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, NP)
Availability in cell line catalogues: ECACC: 93121055, DSMZ: ACC 259
Bibliographic references:
- Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it