HUP-T3

Version 4.0.200811

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HUP-T3 (human, Japanese, pancreas, carcinoma)

SPECIES: human, Japanese
TISSUE/ORGAN: pancreas
TUMOR: carcinoma

DSMZ catalog code: ACC 259
Morphology: epitheloid cells growing adherently in monolayers and clusters
Description: human pancreas carcinoma established from the malignant ascites of a 66-year-old Japanese man with pancreas carcinoma in 1984 confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. S. Saito, Toyama Medical and Pharmaceutical University, Toyama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + 1% non-essential amino acids + 1% sodium pyruvate split confluent culture 1:10 once a week using trypsin/EDTA; seed out about 1 x 106 cells/175 cm 2 at 37C with 5% CO2 about 14 x 106 cells/175 cm 2; frozen with 70% medium, 20% FBS, 10% DMSO at about 1.4 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (flat-moded hypodiploid karyotype with 13% polyploidy; 39(36-40)<2n>XY, -6, -8, -9, -10, -12, -13, -17, -17, -19, -20, +3 mar (2 rings), del(4)(p15), add(11)(q22.3;HSR), add(12)(q24;HSR), add(19)(p13), add(21)(p12); gene amplification) * cytogenetics 2 (suggested by large HSR; ch17 nullisomy) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%), desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)

Availability in cell line catalogues: ECACC: 93121055, ICLC: HTL96006

Bibliographic references:

Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916