HT 1376

Version 4.0.200811

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HT 1376 (human, Caucasian, bladder, carcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: bladder
TUMOR: carcinoma

ECACC catalog code: 87032402
Morphology: Epithelial
Description: Human Caucasian bladder carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 60; Karyotype: 2n = 46, hypertetraploid
Derived from a 58 year old caucasian female with a grade 3 transitional carcinoma of the bladder who had not received chemo or radiation therapy. The cells grow in soft agar and are tumorigenic in mice and hamsters. The patent donor had not received chemotherapy or radiation treatment.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1472, IZSBS: BS TCL 70

Bibliographic references:

J Natl Cancer Inst 1977;58:881 - PMID: 191628