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ICLC catalog code: HTL95015
Continuous culture, grown as monolayer
SPECIES: human, Black (female, 31 years old)
TUMOR: carcinoma, epitheloid
Original line: HeLa
Transfected by: pBact. Back To Transfection WebPage
DEPOSITOR: Dr.U. Pfeffer, Molecular biology Unit, IST at ABC, Genoa Italy.
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Available in the following LABORATORY:
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:6 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with G6PD, NP)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293