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ECACC catalog code: 89102001
Description: Rat liver hepatoma
Viruses: See Properties
Depositor: Dr Strous, Department of Cell Biology, University of Utrecht, NETHERLANDS
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: VSV
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Has been infected with a temperature sensitive mutant of VSV which is defective in RNA replication and transcription.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 8543
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293