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SPECIES: rat, AxC
TUMOR: carcinoma, hepatocellular
ECACC catalog code: 89042702
Description: Rat skin Hepatocellular carcinoma
Viruses: See Properties
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Albumin, transferrin, prothrombin Applications: Gene expression analysis.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Saturation density is reached at 15x1000,000 cells/10cm² dish.
Karyotype: 52 chromosomes
From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat (See Nat Cancer Inst Monogr 1964;13:229). Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. [Same as H4-11-E-C3 (85061112)].
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- J Nat Cancer Inst 1961;26:891
- Natl Cancer Inst Monogr 1964;13:229
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293