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ECACC catalog code: 95091902
Transfected by: Transfection Back To Transfection WebPage
Description: Human fibrosarcoma (retroviral packaging cells)
Viruses: See Properties
Depositor: Dr Y Takeuchi, The Institute of Cancer Research, London, UK (Originator)
Restricted. Patent: None Specified By Depositor
Properties: Applications: Packaging of retrovirus vector.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:20 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
FLYRD18 has been developed together with FLYA13 (95091901) for packaging of retrovirus. it is especially useful for in vivo gene delivery. Both cell lines have been derived from HT1080 (85111505). FLYRD18 has been transfected with Moloney murine leukaemia virus cores with envelope glycoproteins of cat endogenous virus RD114. This cell line is able to produce human complement-resistant retroviral vectors at titres consistently higher than 1x10,000,000i.u./ml. Addition of 4µg/ml blasticidin S and 10µg/ml phleomycin into the media may enhance the packaging function. No replication competent virus has been detected in this cell line.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- J Virol 1995;69:7430 - PMID: 7494248
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293