8505C

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

8505C (human, thyroid, carcinoma, undifferentiated)

SPECIES: human
TISSUE/ORGAN: thyroid
TUMOR: carcinoma, undifferentiated

DSMZ catalog code: ACC 219
Morphology: epithelial-like adherent cells growing as monolayer
Description: human thyroid carcinoma established from the primary tumor of a 78-year-old woman with thyroid carcinoma (undifferentiated carcinoma, histologically a largely papillary adenocarcinoma with some spindle, polygonal and giant cells) confirmed as human with IEF of AST, LDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. M. Akiyama, Radiation Effects Research Foundation, Hiroshima, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:5 every 2-3 days or 1:10 every 4-5 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 20 x 10 6 cells/175 cm 2; doubling time of about 30-40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hypotriploid karyotype with 16% polyploidy; 65(57- 68)<3n>XX,-X, -2, +3, -7, -10, +11, -13, -16, -18, -19, +2mar, del(1)(q13), del(3)(q22), ?del(4)(q21), i(8q)) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * reverse transcriptase (reverse transcriptase not detected)

Availability in cell line catalogues: ECACC: 94090184

Bibliographic references:

Cancer Res 1992;52:1369 - PMID: 1737400
Int J Oncol 1994;4:583-586