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TISSUE/ORGAN: peripheral blood, B cell
ECACC catalog code: 93093003
TRANSFORMED BY: Epstein-Barr
Endogenous viruses: contains Epstein-Barr
Description: Human B cell lymphocyte, EBV transformed
Viruses: See Properties
Depositor: Prof M-P Lefranc and Prof G Lefranc, Laboratoire d'Immunogénétique Moléculaire, Universite Montpellier II, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37C.
Peripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletions, at the homozygous state, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion I (del G1-EP1-A1-GP-G2-G4) Gm-Am genotype Gm b; -; -A2m2/Gm b ; -; -A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). The EZZ deletion (deletion I), which encompasses 160 kb involves two highly homologous regions located 13 kb downstream of IGHG3 and 13 kb downstream of IGHG4.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- FEBS Lett 1987;213:231 - PMID: 3104087
- Genomics 1989;5:431 - PMID: 2613231
- Human Genet 1994;93:520 - PMID: 8168828
- Immunodefic Rev 1991;2:265 - PMID: 1905558
- Mol Biol Med 1983;1:207 - PMID: 6438434
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293