EBTr

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

EBTr (bovine, trachea, embryonic)

SPECIES: bovine
TISSUE/ORGAN: trachea, embryonic

ECACC catalog code: 87090202
Morphology: Fibroblast
Susceptibility to viruses: bovine diarrhea virus * herpes simplex * infectious bovine rhinotracheitis * parainfluenza 3 * reovirus 3 * swine fever virus * vesicular stomatitis (Ogden strain)
Description: Bovine fetal trachea
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: BVDV, IBRV and parainfluenza type 3 virus.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 36; Karyotype: 2n = 60
Derived from the trachea of a male bovine foetus. The cell line can be used for isolation, replication and quantitation of BVDV, IBRV and parainfluenza type 3 virus. Growth and morphological characteristics decline beyond passage number 55.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 44, IZSBS: BS CL 39

Bibliographic references:

16° Ann Meeting TCA Abstract 1965
Am J Vet Res 1968;29:1003 - PMID: 4967124
Excerpta Medica 1965;19(sect 1)